Haemophilus influenzae on culture and microscopy Immunosuppressed patient, recently diagnosed with Flu A. Presents after initial improvement with fever. Blood cx +ve on chocolate agar. Gram stain from plate. Who am I? What nutritional requirements help differentiate me from other similar species ? This was H. influenzae It requires heamin (factor X) and NAD (factor V) Hence its growth on chocolate agar which is a variant of the blood agar plate. It contains RBCs that have been lysed by slowly heating to 80°C to release those yummy factors needed for bacti growth Dr. Ahmed Babiker @Ahmed_HBabiker #Haemophilus #hinfluenzae #culture #microscopy #clinical #microbiology