Antinuclear Antibody Testing - Nuclear and Cytoplasmic Patterns Nuclear Patterns: • Homogeneous pattern: - SLE, SSc, AIH, JIA - dsDNA, chromatin, Scl70 (in some cases) • Speckled pattern: - SjS, SLE, DM, SSc, PM/SSc OS, MCTD, UCTD - Smith, Sm/RNP, Scl-70, SSA-52 (Ro52), SSA-60 (Ro60), SSB (La), RNA polymerase III, Ku • Centromere pattern: - SSc, PBC/SSc OS - CENP-A, CENP-B • Nucleolar pattern: - SSc, PM/SSc OS, Raynaud phenomenon, SjS, cancer, other SARD - Fibrillarin, RNA polymerase III, PM/Scl100, Th/To, Scl-70 (in some cases) • Nuclear dots pattern: - PBC, DM, other inflammatory conditions - Sp-100, PML, NXP-2 (MORC3 or MJ) Cytoplasmic Patterns: • Reticular/AMA: - PBC, SSc, PBC/SSc OS, PBC/SjS OS - AMA • Speckled: - SLE, SjS, CTD-ILD, IIM, Raynaud phenomenon - EJ, Jo-1, OJ, PL-7, PL-12, MDA5, ribosomal P, SRP • Discrete dots/GW body-like: - Neurologic conditions, SjS, SLE, RA, PBC, UCTD - Anti-GW182, anti-Su/Ago • Golgi/polar: - SLE, SjS, RA, OSs, cerebellar ataxia - Giantin/macrogolgin, golgin-95/GM130, golgin-160, golgin97, golgin-245 • Rods/rings: - HCV treated with pegylated interferon-α/ribaviron combination therapy - IMPDH2 ARUPConsult @ARUPLabs #ANA #Antinuclear #Antibody #Testing #Nuclear #Cytoplasmic #Patterns #differential #diagnosis #rheumatology
