Amyloidosis - Summary
Group of disorders associated with extracellular deposition of fibrils formed from low-molecular-weight proteins in a B-pleated sheet configuration
Types Of Amyloidosis:
• Primary amyloid amyloidosis (AL): immunocyte dyscrasias
• Secondary amyloid A (AA) → Chronic Inflammatory Conditions (Tuberculosis, Bronchiectasis, Chronic osteomyelitis, Rheumatoid arthritis, Ankylosing spondylitis, IBD)
• Familial amyloidosis
• Senile amyloidosis
• Dialysis-related amyloidosis: (β2-microglobulin)
• Heavy-chain (AH) amyloidosis
Amyloidosis Diagnosis:
1. Clinical suspicion
2. Visualization of the protein
3. Characterization of the precursor
4. Identification of the extent of disease
Amyloidosis Clinical Manifestations:
Renal:
• Nephrotic syndrome
• Proteinuria
• Can detect early if there is a "protein-albumin" dissociation in urine
• Amyloid nephropathy is common in AA and AL
Cardiac:
• LVH, low-voltage EKG, & restrictive cardiomyopathy
GI:
• Diarrhea, malabsorption, and pseudo-obstruction
• Hepatosplenomegaly & hepatic failure
• GI bleeding
Neuro:
• Orthostatic hypotension
• Autonomic neuropathy, Peripheral neuropathy causing paresthesias, muscle wasting
Bone/Joint:
• Amyloid arthropathy
• Carpal tunnel syndrome
Muscles:
• Infiltration of muscles
Skin:
• Periorbital purpura and macroglossia
Nails:
• Dystrophy
Heme:
• ↓ activity of factor X, vascular infiltration with amyloid (AMYLOID ANGIOPATHY)
AL Protein:
Monoclonal B Cell Lymphocyte Proliferation → Increased plasma cells → Immunoglobulin light chains → AL Protein
• AL Amyloid: Immunoglobulin light chains as part of plasma-cell dyscrasias
• K or L Immunoglobulin light chain
AA Protein:
Chronic Inflammatory Conditions/Recurring Inflammation → Macrophage activation → IL-1 & IL-6 → AA Protein
• Circulating inflammatory protein
ATTR Protein:
Transthyretin TTR → Mutant TTR → ATTR Protein
When To Suspect Amyloidosis?
• Severe fatigue
• Nail dystrophy, waxy or thickened skin
• Persistent pleural effusions
• Parenchymal nodules (amyloidomas)
• Hepatomegaly: Bruising
• Primary hypoadrenalism
• Nausea/vomiting, malabsorption
• Poor appetite, unintentional weight loss
• Eyelid swelling
• Glaucoma, floaters, periorbital purpura
• Restrictive cardiomyopathy
• Conduction abnormalities, arrhythmia
• Palpable spleen
• Peripheral neuropathy: Carpal tunnel, neuropathy, orthostatic hypotension "glove and stocking" peripheral neuropathy
Amyloidosis Diagnostic Testing
Blood Tests:
• SPEP with Serum immunofixation
• Free light-chain assay, troponin, NT-proBNP
• Creatinine, albumin, alkaline phosphatase, uric acid
Urine Tests:
• UPEP with Urine Immunofixation
• Tissue biopsy-Congo red
• Amyloid typing
Cardiac Testing:
• EKG
• Echocardiogram with Doppler imaging: Normal-size ventricles with disproportionate atrial enlargement
• MRI of the heart
GI Tests:
• Diagnostic imaging of liver and spleen
• Fecal fat measurements
• Serum carotene levels
Nerve Tests:
• Nerve conduction studies
• Other: Serum amyloid P scintigraphy
Biopsies:
• (Subcutaneous fat, minor salivary glands, or rectal mucosa)
• Congo red: apple-green birefringence under polarized light microscopy
• Immunohistochemical staining: Light-chain nature of the amyloid
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