The most common HIV tests used are based on the detection of three biological markers of HIV infection (see Figure1): - Viral RNA (genetic material), earliest to appear: detected by individual or pooled nucleic acid amplification testing (NAAT) - p24 antigen (a viral core protein), next to appear: detected by 4th generation enzyme immunoassay testing (EIA) - HIV antibody, last to appear: detected by 3rd or 4th generation EIA, point-of-care (PIC) or rapid HIV testing, and Western Blot #Diagnosis #InfectiousDiseases #HIVAIDS #Testing #Windows #Screening #Antibody #Detection #Timeline