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diagnosis microbiology endocarditis galactomannan infections infectiousdiseases morphology pathology bcnie biomarkers infective negative yeasts bacteria bloodculture cardiology causes clinical endemic falsepositives
Causes of blood culture negative infective endocarditis Bartonella spp, Brucella spp, Coxiella burnetii, Chlamydia spp, Tropheryma whipplei,
Causes of blood culture ... Tropheryma whipplei, Fungi ... (Aspergillus, Candida ... #Causes #differential ... #Microbiology #CNIE
Fungal Biomarkers: Comparing Beta-D-Glucan and Aspergillus Galactomannan Beta-D-Glucan: • Polysaccharide cell wall component of many fungi •
Fungal Biomarkers ... component of many fungi ... • Pathogens: Candida ... #Biomarkers #BetaDGlucan ... #diagnosis #comparison
Morphology and Descriptions of Fungal Infections with Hyphae or Pseudohyphae in Tissues Yeasts with Pseudohyphae: •
Consistent with Candida ... other hyaline fungi ... with dematiaceous fungi ... #fungi #infections ... #microbiology #differential
Microorganisms Responsible for Infective Endocarditis Native valves: - Streptococcus viridans - Enterococci - Other streptococci
Gram-negative bacilli - Fungi ... (Candida, Aspergillus ... Histoplasma) - Culture ... Gram-negative bacilli - Fungi ... Pathogens #Diagnosis #Differential
Important Fungal Stains Mycology Lab: • Calcofluor white - Fluorochrome that binds glucans and chitin in fungal
preparation from culture ... cell response to fungi ... doesn't stain most fungi ... staining #diagnosis #fungi ... #fungus #comparison
Blood Culture-Negative Infective Endocarditis (BCNIE) Definition: No etiology found after 3 separate blood cultures remain negative for
fastidious bacteria or fungi ... Tropheryma whipplei, Fungi ... (Aspergillus, Candida ... bacteria) and 18S ( fungi ... Endocarditis #diagnosis #differential
Morphology and Descriptions of Endemic Fungal Infections with Yeasts in Tissues Broad-based budding yeasts: • Description: Broad-based
other hyaline fungi ... with dematiaceous fungi ... #fungi #infections ... #microbiology #differential ... morphology #pathology #atlas
Fungal Biomarkers: Beta-D-Glucan and Aspergillus Galactomannan Beta-D-Glucan: • Polysaccharide cell wall component of many fungi • Pathogens:
component of many fungi ... • Pathogens: Candida ... closely related fungi ... #Biomarkers #BetaDGlucan ... #diagnosis #comparison
Morphology and Descriptions of Endemic Fungal Infections with Yeasts in Tissues Broad-based budding yeasts: • Description: Broad-based
Descriptions of Endemic Fungal ... Cryptococcus, and Aspergillus ... pathology #clinical #atlas ... #fungi #microbiology ... #differential #
1,3-Beta-D-Glucan and Galactomannan Testing Sensitivity Specificity and False Positives 1,3-Beta-D-Glucan • Cell wall component of many fungi
component of many fungi ... • Routine use: Candida ... in BAL does not differentiate ... closely related fungi ... blood products #BetaDGlucan
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