How to Collect Swab Specimens for Monkeypox Testing Swab in Tube (ie Copan FLOQSwab) vs Peel Pouch (ie Puritan) #PUI #MPV #MPX #Monkeypox #swab #collection #procedure #instructions #Diagnosis

Appearance of Monkeypox Primary Inoculation Reactions #Monkeypox #MPV #MPX #lesions #clinical #Inoculation #photo

Monkeypox - Virology Causative Agent: Monkeypox virus (MPV) Year of Discovery: 1958 Genome: Double-stranded DNA Variants and Genotypes: 2 clades Primary Route of Transmission: Direct, prolonged contact with monkeypox rash, scabs or bodily fluids from infected person. Potential Animal Reservoirs: Monkeys, rodents, other small mammals. #MPX #MPV #monkeypox #COVID19 #comparison #virology #Diagnosis

Multisystem Inflammatory Syndrome in Children (MIS-C) #MISC #COVID #pediatrics #diagnosis

ACIP recs: - ACAM2000 is a clonal derivative of Dryvax  -Extensive data about Dryvax helped inform recommendations for ACAM2000 - JYNNEOS® is different from ACAM2000  -Recommendations for JYNNEOS® will need to rely on data specifically about JYNNEOS® #Vaccines #ACAM2000 #JYNNEOS # Stockpile #MonkeyPox #Prevention #ACIP #Orthopox

“ Clinical laboratories should follow these guidelines when collecting specimens for monkeypox virus: Collect at least two dry swabs from the same lesion. Swab or brush lesion vigorously with two separate sterile dry swabs. Use a sterile nylon, polyester, or Dacron swab with a plastic, wood, or thin aluminum shaft. Do not use other types of swabs. Place swabs in individual sterile containers. Do not add any transport media. Refrigerate (2–8°C) or freeze (-20°C or lower) specimens within an hour after collection. Store refrigerated specimens for up to 7 days and frozen specimens for up to 60 days. Send refrigerated specimens within 7 days of collection; ship frozen specimens within 60 days of collection. Specimens that are greater than 8°C upon receipt will be rejected. Ship on dry ice as category B. Send both swabs to the state or territorial public health laboratory. All specimens should be sent through the state or territorial public health department, unless authorized to send them directly to CDC. A state public health laboratory may test one of the paired dry swabs for presumptive results. CDC can provide monkeypox virus-specific testing on the second dry swab specimen if the first dry swab is non-variola orthopoxvirus positive at the state or territorial public health laboratory. Laboratories with specimens that are suspected to contain monkeypox virus should contact their state or territorial public health department (contact list) or the CDC Emergency Operations Center (EOC) at 770-488-7100 for guidance” https://cdn.ymaws.com/www.cste.org/resource/resmgr/poc/epioncall_update1622.pdf?ACSTrackingID=USCDC_2146-DM83288&ACSTrackingLabel=Lab%20Advisory%3A%20CDC%20Specimen%20Collection%20Guidelines%20for%20Monkeypox%20Virus&deliveryName=USCDC_2146-DM83288 #Laboratory #SpecimenCollection #ClinicalLaboratory #Clinicipathology #MonkeyPox #OrthoPox

6/02/2022 UPDATE: This assay is designed to detect non-variola orthopoxviruses, including monkeypox virus. It is not intended to specifically detect monkeypox virus or to differentiate monkeypox virus from the other orthopoxviruses this assay detects. https://www.cdc.gov/csels/dls/locs/2022/06-02-2022-lab-advisory-CLARIFICATION-CDC_Publishes_Non-variola_Orthopoxvirus_PCR_Testing_Procedure_1.html 05/30/2022 “Physicians should use this algorithm to categorize a patient’s risk of smallpox –low, moderate, or high– when they present with an acute, generalized vesicular or pustular rash illness. Report a suspected case of smallpox immediately to the appropriate local, state or territorial health department.If, after their review, the patient meets the criteria for high-risk of smallpox, the case should be immediately reported to the CDC Emergency Operations Center (770-488-7100). https://www.cdc.gov/smallpox/pdfs/smallpox-diagnostic-algorithm-poster-2-pages.pdf *Real-time polymerase chain reaction (PCR) is the preferred method for detecting variola virus* PCR testing on specimens from low- to moderate-risk patients is available across the United States through reference level Laboratory Response Network (LRN) member laboratories. Variola virus-specific PCR testing on specimens from high-risk patients is limited to select reference level LRN member laboratories meeting additional facility and vaccination requirements and the CDC. CDC should be consulted prior to initiation of any testing on high-risk specimens. The type of specimen collected will vary depending upon disease progression. See table below for a list of acceptable specimens. Whole blood alone is not a suitable specimen for smallpox diagnosis by PCR as viremia generally concludes with rash onset. Although electron microscopy and antibody detection can aid in diagnosis, these assays alone are not definitive for variola virus. Review the Negative Staining Electron Microscope Protocol for Rash Illness” *** Report all HIGH RISK CASES immediately (without waiting for lab results) to hospital infection control and applicable public health department*** Additional resources: https://www.paho.org/en/file/109251/download?token=kCLnmgNR #SmallPox #Laboratory #Diagnosis #Clinicopathology