19 results
Important Fungal Stains
Mycology Lab:
 • Calcofluor white - Fluorochrome that binds glucans and chitin in fungal
Important Fungal ... preparation from culture ... • India ink - "Negative ... #microbiology #microscopy ... #fungus #comparison
Causes of blood culture negative infective endocarditis

Bartonella spp, Brucella spp, Coxiella burnetii, Chlamydia spp, Tropheryma whipplei,
negative infective ... Tropheryma whipplei, Fungi ... BCNIE #Causes #differential ... InfectiousDiseases #Microbiology ... #CNIE #BCNIE #cardiology
Microorganisms Responsible for Infective Endocarditis

Native valves:
 - Streptococcus viridans 
 - Enterococci 
 - Other streptococci
bacilli - Fungi ... Histoplasma) - Culture ... bacilli - Fungi ... streptococci - Culture ... Pathogens #Diagnosis #Differential
Causes of Culture Negative Abscess - Differential Diagnosis Framework
Consider prior antibiotics as a cause of a
Causes of Culture ... Negative Abscess ... - Differential ... culture Brain: ... #Differential #
Endocarditis Algorithm - Infective vs Noninfective Causes
Noninfective:
 • Malignancy
 • Autoimmune disease
 • Systemic infection
Native Valve
• HACEK • Culture-negative ... epidermidis • Gram-negative ... bacilli • Fungi ... bacilli • Fungi ... Noninfective #Causes #Differential
Blood culture contaminants
Grouped by gram negative vs gram positive vs other as well as never/maybe/probably
Some additional
Blood culture contaminants ... Grouped by gram negative ... #Infections #Microbiology ... #Micro #Differential
CAPD-associated peritonitis (Continuous Ambulatory Peritoneal Dialysis) - 5 Things To Know
Diagnosis (Two of the following):
(1) Clinical
Positive effluent culture ... Microbiology: • ... • 15-35% Gram negative ... negative peritonitis ... prior abx use, fungal
Blood Culture-Negative Infective Endocarditis (BCNIE)
Definition: No etiology found after 3 separate blood cultures remain negative for
Blood Culture-Negative ... remain negative ... fastidious bacteria or fungi ... Tropheryma whipplei, Fungi ... Endocarditis #diagnosis #differential
Diarrhea Workup and Management Algorithm in Transplant Patients
First Tier Microbiologic-Testing:
1. C. difficile PCR (Stool)
2. CMV qPCR
Patients First Tier Microbiologic-Testing ... Bacterial culture ... pathogens First Tier Microbiologic-Testing ... above testing negative ... Transplant #diagnosis #differential
Fungal Biomarkers: Beta-D-Glucan and Aspergillus Galactomannan
Beta-D-Glucan:
 • Polysaccharide cell wall component of many fungi
 • Pathogens:
Fungal Biomarkers ... Coccidioides - Negative ... Cryptococcus - Negative ... BAL GM does not differentiate ... Galactomannan #diagnosis #comparison